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| |
Experiment Two
Spring Treatment of Nosema using Fumigillan in Pollen Patties
Experimenter: Eric
Abell
|
| Objective: to
determine if it is advantageous to feed fumigillan in patties in the spring. |
Method: Pollen
supplement patties were made with and without fumigillan in the recipe. As I visited each
yard, a 1 lb (approx) patty was placed on the top bars of all colonies. I selected 1
pallet and collected fees from the underside of the inner pillow from all 4 colonies (or 3
if there were only 3 available). I intend to sample these same colonies in a few weeks.
Question: Will there be a difference between those colonies
that received fumigillan in the patty and those that did not? If so, how much? |
Nosema
spore count before Fumigillan patty application
|
| # |
Millions of
Spores/bee |
Treatment
(See Note) |
| 1 |
4.00 |
N |
| 2 |
40.00 |
N |
| 3 |
0.05 |
N |
| 4 |
0.00 |
N |
| 5 |
6.00 |
N |
| 6 |
0.00 |
N |
| 7 |
4.00 |
N |
8
|
0.05 |
N |
| 9 |
1.20 |
N |
| 10 |
0.05 |
N |
| 11 |
0.65 |
N |
| 12 |
0.90 |
N |
| 13 |
0.00 |
D |
| 14 |
0.00 |
D |
| 15 |
0.00 |
D |
| 16 |
0.05 |
D |
| 17 |
4.75 |
Y |
| 18 |
0.00 |
Y |
| 19 |
4.75 |
Y |
| 20 |
0.00 |
Y |
| 21 |
0.00 |
Y |
| 22 |
15 or 0 |
Y |
| 23 |
0.00 |
Y |
| 24 |
15 or 0 |
Y |
| 25 |
0.00 |
Y |
| 26 |
0.00 |
Y |
| 27 |
0.00 |
Y |
| 28 |
0.05 |
Y |
| 29 |
0.00 |
Y |
| 30 |
0.00 |
Y |
| 31 |
? |
Y |
| 32 |
0.00 |
Y |
| 33 |
20+ |
Y |
| 34 |
0.00 |
Y |
| * = these samples may
have been switched |
| Fumigillan Dose in Patties: |
D = 9.5 g /100 colonies |
|
|
Y = 4.75 g/100 colonies |
|
|
N = None |
|
Nosema 98
|
before patty:
|
Fumigillan
|
at
pollen
|
reduced
|
|
|
million
|
Yes/No
|
flow
|
|
|
|
spores/bee
|
Double
|
million
|
|
|
|
|
|
spores/bee
|
|
1
|
Kalisvart
|
4.00
|
N
|
2.5
|
|
2
|
Derouin
|
40.00
|
N
|
0
|
|
3
|
Vischer (W)
|
0.05
|
N
|
4
|
|
4
|
Vischer (S)
|
0.00
|
N
|
|
|
5
|
Vischer (N)
|
6.00
|
N
|
|
|
6
|
Yakimetz
|
0.00
|
N
|
|
|
7
|
Schwing
|
4.00
|
N
|
0
|
|
8
|
Hein
|
0.05
|
N
|
|
|
9
|
Amelia
|
1.20
|
N
|
|
|
10
|
Sharron
|
0.05
|
N
|
1
|
|
11
|
Pasay (Vic)
|
0.65
|
N
|
0.05
|
|
12
|
Pasay (S)
|
0.90
|
N
|
1.45
|
|
13
|
Kuchmak
|
0.00
|
D
|
|
|
14
|
Pasay (N)
|
0.00
|
D
|
|
|
15
|
Kotyk
|
0.00
|
D
|
|
|
16
|
Weeks
|
0.05
|
D
|
|
|
17
|
Rozak
|
4.75
|
Y
|
|
|
18
|
McRobbie
|
0.00
|
Y
|
|
|
19
|
Walters
|
4.75
|
Y
|
0
|
|
20
|
Andersen
|
0.00
|
Y
|
|
|
21
|
Andersen-E
|
0.00
|
Y
|
|
|
22
|
Abramski *
|
15 or 0
|
Y
|
|
|
23
|
Andersen-W
|
0.00
|
Y
|
|
|
24
|
Milligan *
|
15 or 0
|
Y
|
0.6
|
|
25
|
Scopick
|
0.00
|
Y
|
|
|
26
|
Schafer
|
0.00
|
Y
|
|
|
27
|
Tuckey
|
0.00
|
Y
|
|
|
28
|
Pasnak
|
0.05
|
Y
|
0
|
|
29
|
Thereux
|
0.00
|
Y
|
|
|
30
|
Prins (S)
|
0.00
|
Y
|
|
|
31
|
Deveaux
|
?
|
Y
|
|
|
32
|
Goutbeck
|
0.00
|
Y
|
|
|
33
|
Prins (E)
|
20+
|
Y
|
2.35
|
|
34
|
Boisjoli
|
0.00
|
Y
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
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|
Note Fumigillan D = 9.5 g /100
colonies Y = 4.75 g/100 colonies
* these samples may have been switched
|