From: James C. Bach
Date: 3/9/98
Time: 7:18:40 PM
Remote Name: 206.129.139.38
May I kindly correct you? I analyzed your post and technique. I had no intent to criticise.
I can't recall having taken two 25 bee samples from the same bee sample. My haemocytometer had two grids. I remember that often the reading from each grid was different from the other by as much as ten spores or so. As I recall one spore on the entire grid equals 4,000 spores per bee so finding one spore on one grid and five on the other creates a considerable difference.
Another thing I recall is that I only focused the microscope until I found the spores. Sometimes if I focused down to the bottom of the sample I would pick up a few more spores and more debris. It has been a while since I have performed the procedure.
On occasion I found round structures in the field of view with the Nosema spores. One needs to be careful not to count these. Nosema spores are quite distinctive, I think.
Regards,
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